Analysis Instruments

HPLC Column Storage Cabinet provides efficient spacing to store 120 HPLC columns. HPLC Column is designed exclusively with the industrial grade to separate and analyze the individual component and part of chemical mixtures. The HPLC columns offers high surface area thus improving the overall separation and purification of individual components in its purest form. The high-quality column has small and a narrow particle size making the purification and separation process far more efficient in comparison to any other material. The cabinets has a wide variety of applications in pharmaceutical, molecular biology and environmental sciences.

An HPLC Solvent Bottle Safety Cap is a specialized cap designed to securely seal solvent bottles used in High-Performance Liquid Chromatography (HPLC) systems. These caps play a critical role in ensuring the safe and efficient storage and handling of solvents in a laboratory environment, while also helping to maintain the integrity of the HPLC system.

HPLC Safety Bottle Caps
HPLC Bottle Cap As Per (ISO Standard Inc A-620) Port -2,3,4
Material : White Tefzel Insert ETFE 9 ( Ethylene tetrafluoroethylene )
Cap Size : GL45.
Port Size : 1/8 with Vent.

Short Specification:

Hydrophilic membrane filtration is used in several industries to filter various liquids. These hydrophilic filters are used in the medical, industrial, and biochemical fields to filter elements such as bacteria, viruses, proteins, particulates, drugs, and other contaminants.

Aone Safety-Caps offers a safe and reliable protection against harmful Solvent vapors. The Safety Air Inlet Valve has a check Valve which blocks harmful solvent vapors and keep the mobile phase composition more stable. A Syringe Filter on top of the Safety Air Inlet Valve offers a protection against contamination of dust and particles from the incoming air.

Other Details:

• Available in Nylon, PVDF, PTFE & GF
• Color coded for easy identification
• Polypropylene housing
• Reusable storage container
• Cost-effective

Specifications:

• Pore Size : 0.22 um, 0.45 um
• Diameter : 13 mm, 25 mm & 33 mm

High Pressure Fittings Offer Extra Durability. They Are More Robust To Higher Temperatures, Solvent Exposure And OverTightening Than Standard PEEK Fittings. They Are Available In A Variety Of Dimensions, All With Built-In Ferrules.

Handle HPLC Chemical Waste with Care

Sealed safety cans and carboys with leak-proof systems ensure HPLC waste stays where it belongs. Coalescing carbon filters even make sure the vapors pose no risk to laboratory workers.

Peek Ferrules provide secure seals between chromatography columns and injectors and detectors; These are often used in high pressure applications.

Details:

• PEEK Column Coupler delivers a low swept-volume connection while maintaining column efficiency.
• Our couplers are an excellent option for reducing overall flow path volume while coupling two devices together.
• It is manufactured from biocompatible PEEK giving is excellent chemical resistance to virtually all commonly used solvents.
• It has a swept volume of 3.6uL, a thru-hole of .010″ and a male to male thread configuration of 10-32 Coned.

A PEEK Union is a specialized connector fitting made from PEEK (Polyetheretherketone), a high-performance thermoplastic polymer known for its chemical resistance, durability, and high-temperature tolerance. The PEEK Union is used to securely join two pieces of tubing or pipes, especially in applications that require high purity, resistance to harsh chemicals, and resistance to elevated temperatures.

Short Specification:

Peek Union
HPLC PEEK union for 0.25mm I.D. tubing.
1. Easy connection of transfer/solvent lines
2. Low dead volume
3. Completely inert and biocompatible

Product Description

Our PEEK Tees and Crosses are precisely designed to withstand environmental stresses in the high-pressure regions of a system. It is manufactured from inert PEEK, which delivers excellent chemical resistance.

Featured are our PEEK Micro Tees and Crosses that join capillary tubing together. Several researchers use our PEEK Micro Tee to introduce fluid to their stream just prior to a Mass Spectrometer because of its advantageous internal geometry and PEEK polymer’s electrical resistance.

Designed for secure liquid-tight connections Adapters for semi-rigid tubing are color-coded by tubing size for easy identification. A two-piece nut and ferrule system provides a liquid-tight connection and withstands pressures up to 500 psi.

Product Description

Peek Ferrules provide secure seals between chromatography columns and injectors and detectors; These are often used in high pressure applications.

Our 7 Port PEEK Manifold Assembly can be easily applied for a multi-Ports mixing chamber, combining several fluid streams into one or one stream split to several outlets.It can also be used as a gas sparging splitting unions or sample injection onto multi-well plates or a multiple direction flow path union.

Our manifold is manufactured from biocompatible PEEK giving it excellent chemical resistance. It has 7 Ports and is for 1/16″OD tubing.

1. Material Composition:

• Filter Holder Material:
  • Stainless Steel: Durable, resistant to corrosion, and suitable for high-pressure applications. Common for reusable pre-column filter holders.
  • Polypropylene (PP): Lightweight, cost-effective, and chemically resistant, often used in disposable filter holders.
  • Glass: Transparent, providing visibility of the filtering process and highly resistant to pressure.
• Frit Material:
  • Stainless Steel: Commonly used for frits due to its durability and resistance to pressure.
  • PEEK (Polyether Ether Ketone): Used in high-performance applications due to its excellent chemical resistance and mechanical strength.
  • PTFE (Polytetrafluoroethylene): Chemically inert, used for compatibility with a wide range of solvents, especially aggressive or non-aqueous solvents.
  • Nickel Plated Steel: Used in specific frits where high strength is needed, particularly in high-pressure applications.

2. Design and Dimensions:

• Filter Holder Sizes: Available in various sizes depending on the chromatography system, such as:
  • 10 mm, 20 mm, 25 mm, 47 mm, 50 mm, and larger sizes.
• Frit Dimensions: Typically designed to match the size of the filter holder. Common frit sizes are 10 mm, 25 mm, 47 mm, or 50 mm.
• Pore Size:
  • 0.2 µm: Fine filtration, used in high-performance chromatography applications to remove bacteria and fine particles.
  • 0.45 µm: Standard size for general filtration of particulate contamination.
  • 1 µm and larger: For pre-filtration of larger particulates or coarse filtration before introducing the solvent to the column.
• Flow Rate: Filter holders are designed to handle varying flow rates, generally ranging from 50 mL/min to 200 mL/min, depending on the filter size and solvent viscosity.
• Operating Pressure: Commonly designed to withstand pressures up to 10 bar (145 psi), though higher pressures may be accommodated with specific materials and designs.

3. Applications:

• Chromatography Systems (HPLC, UHPLC): Used to filter the mobile phase before it enters the chromatography column, preventing column fouling and improving performance.
• Ion Chromatography: Ensuring that the ion-exchange column is protected from particulate contamination in the mobile phase.
• Pharmaceutical and Biopharmaceutical Analysis: Ensures that mobile phases are free of particulates which could interfere with sensitive biological assays.
• Environmental Testing: Filtering solvents and samples for analysis in water, air, or soil testing.
• Food & Beverage Testing: Used to filter solvents and mobile phases before testing food and beverage components.
• Chemical Analysis: Protecting the analytical columns from contamination and preserving the integrity of the chromatography results.

A Stainless Steel Filter Holder is a laboratory device used to support and secure membrane or other types of filters during liquid or gas filtration processes. It provides a rigid and leak-proof housing that allows fluids to pass through the filter media under vacuum or pressure, ensuring reliable separation of particles, microorganisms, or contaminants.

Key Features:

• Material: Made of high-grade stainless steel (usually SS 316/316L) for chemical resistance, durability, and easy cleaning.
• Filter Compatibility: Designed for membrane filters (commonly 47 mm, 90 mm, or 142 mm diameter, depending on the model).
• Design: Consists of a base with a fritted support (porous stainless steel or glass), a filter holder cup, clamp or spring locking system, and connections for vacuum/pressure lines.
• Sterilization: Autoclavable and resistant to repeated sterilization cycles.
• Sealing: Equipped with silicone or PTFE gaskets to prevent leakage.

Applications:

• Microbiological Testing: Filtration of water, beverages, or pharmaceutical samples for microbial contamination.
• Particulate Analysis: Determining suspended solids in liquids.
• Sterility Testing: In pharmaceutical quality control labs.
• General Laboratory Filtration: For sample clarification, purification, and analytical testing.

Types:

1. Vacuum Filter Holder – Uses vacuum suction below the filter to draw liquid through.
2. Pressure Filter Holder – Uses applied pressure above the sample for filtration.
3. Multi-Branch Manifold Filter Holders – Allow simultaneous filtration of multiple samples.

👉 Common Sizes: 25 mm, 47 mm, 90 mm, 142 mm filter holders.

Peek Coupler 
PEEK Column Coupler delivers a low swept-volume connection while maintaining column efficiency. Our couplers are an excellent option for reducing overall flow path volume while coupling two devices together. It is manufactured from biocompatible PEEK giving is excellent chemical resistance to virtually all commonly used solvents. It has a swept volume of 3.6uL, a thru-hole of .010" and a male to male thread configuration of 10-32 Coned.

Short Specification:

An HPLC column oven is a thermostatted enclosure designed to house and maintain a precise and uniform temperature around chromatography columns during high-performance liquid chromatography. Its purpose is to enhance separation consistency, reproducibility, and resolution by mitigating external temperature fluctuations and internal viscous heating effects.

Why Temperature Control Matters in HPLC

·         Retention time stability: In isocratic reversed-phase LC, retention times can change by 1–2% per 1 °C variation—impacting separation accuracy.

·         Viscous friction heating: High pressures (especially in UHPLC) generate internal heat within the column, potentially causing peak distortion or decreased efficiency.

·         Preventing thermal gradients: Temperature inconsistencies across the column radius can reduce separation efficiency and introduce baseline noise or ghost peaks.

Core Components & Features

1.       Heating & Cooling Mechanisms:- Options include block heaters, forced-air systems, or Peltier (thermoelectric) modules for both heating and cooling (no refrigerants).

2.       Precision Control (PID Technology):- Digital PID controllers offer accurate temperature regulation, typically within ±0.1–0.2 °C. Stability follows closely—also around ±0.1–0.2 °C. Some systems provide fuzzy PID or advanced algorithms for enhanced stability.

3.       Uniformity & Distribution:- Internal fans or conduction blocks ensure even temperature across the entire column. Uniformity often maintained within ±0.2–0.5 °C.

4.       Temperature Range & Ramp:- Typical range spans from just above ambient to ~80–100 °C. Some models cool below ambient (e.g., down to 4 °C). :- High-end models support sub-ambient cooling with Peltier modules.

5.       Safety & Monitoring:- Include leak detection, over-temperature protection, sensor failure alerts, and sometimes liquid spill drainage.

6.       Programmability & Interfaces:- Many ovens offer time/temperature programming, countdown timers, and auto-shutoff. Interfaces like RS‑232 enable remote control or logging.

7.       Column Accommodation:- Designed to hold 1–4 columns (usually up to 30 cm long), including options with larger diameter support

 Laboratory Glass Filter Holder

Used for vacuum or pressure filtration, especially in microbiology and analytical chemistry.

Typical components:

• Glass funnel (for the sample)
• Filter support base (with a fritted glass or membrane support)
• Clamp or metal holder
• Vacuum outlet (for suction)
• Flask or receiver below

Used for:

• Filtering particles or microbes from liquids
• Sterile filtration using membrane filters (e.g., 0.22 µm)
• Gravimetric analysis

Example: Millipore, Whatman, or Pyrex glass filter holders.

Photographic Glass Filter Holder

Used in cameras or photography lighting setups to hold square/rectangular glass filters in front of a lens.

Features:

• Mounts onto the front of a lens
• Holds ND filters, color correction filters, or polarizers
• Usually used in landscape photography

Materials: Often metal or plastic holder with glass or resin filters.

3. Air Sampling Glass Filter Holder

Used in environmental testing to collect particulate matter or aerosols from air.

Features:

• Made of borosilicate glass
• Holds filter membranes (often quartz or fiberglass)
• Connects to air sampling pumps.

 4. Water Filtration Glass Filter Holder

Used to hold glass fiber or membrane filters for filtering water samples, often in environmental or pharmaceutical labs.

Key features:

• Autoclavable borosilicate glass
• Stainless steel or aluminum clamps
• Used with vacuum filtration setups.

 Things to Consider When Buying One:

• Filter size (e.g., 25 mm, 47 mm, 90 mm)
• Compatibility with membrane types
• Chemical resistance
• Auto clability (for sterile applications)
• Vacuum pressure rating

COLUMN END PLUGS
COLUMN END PLUGS Column end plugs are used to prevent HPLC columns from drying out when not in use. Made of hard nylon, these end stoppers are available in packs of 10 and in different colors.
• 49027-01B End plugs for HPLC columns. Nylon 10-32 Thread. White. 100/Pack.
• 49027-06K End plugs for HPLC columns. Nylon 10-32 Thread. Green. 100/Pack.
• 49027-04W End plugs for HPLC columns. Nylon 10-32 Thread. Red 100/Pack.
• 49027-06K End plugs for HPLC columns. Nylon 10-32 Thread. Black. 100/Pack.
• 49027-04W End plugs for HPLC columns. Nylon 10-32 Thread. Blue . 100/Pack.

Short Specification:

·         Column End Plugs are essential accessories designed to securely seal both ends of chromatography columns, preventing contamination, moisture ingress, and solvent evaporation during transport, storage, or after column packing. These plugs ensure the column’s internal packing remains stable, clean, and uncontaminated before use or between runs, extending the overall lifespan and maintaining performance integrity.

·         Manufactured from high-performance materials such as PEEK, PTFE, or polypropylene, our column end plugs offer excellent chemical resistance, ensuring compatibility with a wide range of solvents and reagents commonly used in HPLC, UHPLC, and flash chromatography. Available in multiple sizes and thread types (including standard 10-32 and ¼-28 threads), they fit most column brands and configurations with ease.

·         Each plug is precision-molded or machined for a tight, leak-proof seal, ensuring zero cross-contamination and preventing sample loss or degradation. They are ideal for use in pharmaceutical labs, chemical analysis labs, biotech facilities, and academic research environments. Whether you're storing columns long-term or protecting them during shipment, column end plugs are a simple yet critical solution to maintain system integrity.

1. Material Composition:

• PEEK Tubing:

  • Polyether Ether Ketone (PEEK): A high-performance thermoplastic known for its exceptional chemical resistance, high thermal stability, and mechanical strength.
  • Properties:
    • Excellent resistance to acids, bases, solvents, and other aggressive chemicals.
    • High temperature resistance (up to 260°C or 500°F).
    • Low friction, making it ideal for fluid handling.
    • Biocompatible, making it suitable for medical and pharmaceutical applications.
    • Non-conductive.

• SS Tubing (Stainless Steel):

  • Stainless Steel (typically 304 or 316L): Known for its corrosion resistance, durability, and strength.
  • Properties:
    • Excellent resistance to corrosion, especially against chloride and other aggressive chemicals.
    • High tensile strength and pressure tolerance.
    • Can withstand high temperatures (up to 800-1000°C depending on the grade).
    • Easy to clean and sterilize, making it suitable for sanitary applications.
    • Magnetic properties (varies based on grade).

2. Design and Dimensions:

• PEEK Tubing:

  • Inner Diameter (ID): Typically ranges from 0.2 mm to 6 mm, depending on the application.
  • Outer Diameter (OD): Commonly available in 1 mm, 2 mm, 3 mm, 4 mm, and larger sizes.
  • Wall Thickness: Standard wall thicknesses are 0.25 mm, 0.5 mm, and 1 mm.
  • Length: Customizable lengths ranging from 1 meter to 100 meters.
  • Color: Generally natural (transparent), but can be available in different colors for identification.

• SS Tubing:

  • Inner Diameter (ID): Typically ranges from 1/16 inch (1.6 mm) to 1 inch (25.4 mm), with various other sizes in between.
  • Outer Diameter (OD): Common sizes include 1/16 inch, 1/8 inch, 1/4 inch, 3/8 inch, and 1/2 inch.
  • Wall Thickness: Ranges from 0.020 inch to 0.065 inch, depending on pressure and flow requirements.
  • Length: Custom lengths available from 1 meter to 100 meters or more, depending on the supplier.

3. Applications:

• PEEK Tubing:

  • Chromatography: Widely used in HPLC (High-Performance Liquid Chromatography) for connecting components in a system due to its chemical and pressure resistance.
  • Medical and Pharmaceutical: Due to its biocompatibility, PEEK is used in applications such as drug delivery, medical devices, and instrumentation.
  • Fluid Handling Systems: Ideal for carrying aggressive or high-temperature fluids in laboratory or industrial settings.
  • Electronics: Used for insulation purposes in electronic applications due to its high dielectric strength.
  • Aerospace: Used in fuel and lubrication lines due to its temperature and pressure resistance.